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KMID : 0377519910160040431
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Chung-Ang Journal of Medicine
1991 Volume.16 No. 4 p.431 ~ p.452
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An Experimental Study for the Effects of Pseudomonas aeruginosa, Serine Prote-ase and Aprotinin in the Rabbit Cornea
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Abstract
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In this experimental study we had injected Pseudomonas extract, Serine protease and Aprotinin (protease inhibitor) into the corneal stroma of rabbit.
For this study we used 44 albino rabbits and divided them into 6 groups according to the injection materials as below:
A : Extract of Pseudomonas
B : Extract with Aprotinin 20DO u
C : Serine protease
D : Serine protease with Aprotinin 500 u
E : Serine protease with Aprotinin 2000Q
F : Balanced salt solution (Control)
After the injection of each sample we examined the degree of corneal opacity, presence of descemetocele and corneal perzoration and the degree of anterior chamber reactions as cells and flares with time interval(1 hour to 1 week) using the slit-lamp biomicroscope.
The corneal endothelial thickness and endothelial cell density was also checked with the specular microscope.
Fot the histopathologic examination we enucleated every samples with time interval and made specimens for light and electron. microscope.
The results were as follows :
1. The degree of anterior chamber reaction(cells & flares) was more marked in the serine protease group with peak reaction from 10 hours to 24 hours (p < 0.05) .
2. The corneal opacity in the Extract group was more marked (+2.1´ than combined A pratinin 200Qu injection group ( +0.9) (p<0.01).
3. The descem toce,e formation was observed in Extract group and serine protease group, and the occurrent :-ate was 52.5%, 50.% respectively after 48 hours.
The corneal rforation was developed 3 eyes in Extract group within 10 hours after injection
4. Under the s ec-alar microscopic examination the increment of corneal thickness secondary to stromal edemp., and marked endothelial cell loss were observed in Extract group and serine pr0-tease group conloared with control group (p<0.0i).
5. The histopathological changes exhibits loosening of stromal collagen fibers, diffuse intracelluar edema, endothelial degeneration associated with fibrinol:d membrane formation in Extract group and serine protease group.
6. Electron-lucf=_? area with -loss of web-like pattern of the stromal proteoglycan ground sub-stance was more marked in Extract group and serine protease group.
Thus we could na ke a conclusion that combined injection of Aprotinin with Pseudomonas extract or serine protean ,ras more effective preventing the early period of Pseudomonas infection from corneal damage c __:spared with a single injection of Extract or serine protease.
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